rabbit anti human rab7  (Santa Cruz Biotechnology)

Journal: EBioMedicine

Article Title: CRISPR-Cas9 Mediated Exonic Disruption for HIV-1 Elimination

doi: 10.1016/j.ebiom.2021.103678

Figure Lengend Snippet: CRISPR LNPs Cell Trafficking. Rhodamine conjugated DHPE (1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine) phosphoglycerol tracked the locale of CRISPR LNPs in human MDMs. Confocal microscopy was employed 12 h after LNPs injection in the MDM cultures. Alexa-Fluor 488 (green) secondary antibody detected Rab 5, Rab7, or Lamp1 subcellular compartments. Phalloidin-iFluor 647 marked cell boundaries. The MDM nucleus was stained with DAPI (blue). Rhodamine DHPE phospholipid containing CRISPR-LNPs (red) colocalized with Rab5 (a) and Rab7 (green) (b). (c) No colocalization was found between Lamp1 (green) and the nanoparticles (red). (d) TM-Rhodamine labeled px333DE was used for CRISPR LNPs to examine nuclear localization of the CRISPR payload present in the nucleus 12h after treatment. Z-stack affirmed that the CRISPR reached the nucleus.

Article Snippet: The following primary antibodies were used to label different endosomal compartments: Rabbit anti-human Rab5, (Abcam #ab18211), early endosomal marker, rabbit anti-human Rab7 (Santacruz biotechnology #sc10767), a late endosome marker, and rabbit anti-human Lamp-1 (lysosomal-associated membrane protein-1) (Abcam #ab24170) for lysosomes.

Techniques: CRISPR, Confocal Microscopy, Injection, Staining, Labeling

Journal: eLife

Article Title: A fusion peptide in preS1 and the human protein disulfide isomerase ERp57 are involved in hepatitis B virus membrane fusion process

doi: 10.7554/eLife.64507

Figure Lengend Snippet: Huh7-NTCP cells were grown on glass cover slides and fixed 48 hr after seeding. ( A ) Endogenous ERp57 with Rab5, Rab7, Rab11, or Lamp1 were immune-stained, and the colocalization of ERp57 (red channels) with Rab5, Rab7, Rab11, or Lamp1 (green channels) was analyzed by confocal microscopy. Scale bars of panels and zooms from squared area represent 10 µm and 2 µm, respectively. ( B ) The degree of colocalization between ERp57 and the different cell markers was assessed by determining the Pearson’s correlation coefficients with the JACoP plugin of ImageJ. Results are expressed as the mean of six individual cells. Error bars correspond to standard deviations.

Article Snippet: Antibody , Anti-human Rab7 (rabbit monoclonal) , Cell Signaling Technology , (D95F2):9367 , IF (1:100).

Techniques: Staining, Confocal Microscopy

Journal: eLife

Article Title: A fusion peptide in preS1 and the human protein disulfide isomerase ERp57 are involved in hepatitis B virus membrane fusion process

doi: 10.7554/eLife.64507

Figure Lengend Snippet:

Article Snippet: Antibody , Anti-human Rab7 (rabbit monoclonal) , Cell Signaling Technology , (D95F2):9367 , IF (1:100).

Techniques: Virus, Produced, Variant Assay, Construct, Generated, Transduction, Retroviral, Plasmid Preparation, Selection, shRNA, Transfection, Expressing, Clone Assay, Luciferase, Isolation, Sequencing, Mutagenesis, Reporter Assay, Activity Assay, cDNA Synthesis, SYBR Green Assay, Membrane, Integrity Assay, Cytotoxicity Assay, Software, Staining, RNA Extraction